Oligonucleotide Synthesis: Methods and Applications Herdewijn Piet
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective bi… Celý popis ↓
Porovnanie cien Oligonucleotide Synthesis: Methods and Applications Herdewijn Piet
Popis Oligonucleotide Synthesis: Methods and Applications Herdewijn Piet
Oligonucleotide Synthesis: Methods and Applications Herdewijn Piet
A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation.
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